As a biochemical marker of brain injury, S100-B protein is closely related to the degree and prognosis of brain injury, and its stability is good, and the detection of its concentration value is helpful to clinically judge the lesion size, treatment effect and prognosis of nerve tissue. Therefore, the determination of the level of S100- 3 protein can provide an effective basis for clinical judgment and triage of patients' disease types and follow-up series of treatment methods, saving a lot of valuable time.